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	Provedor de dados OAK (1) Electron. J. Biotechnol. (1) Autor Agungpriyono, Dewi Ratih (1) Furuoka, Hidefumi (1) Kim,Jong-Wan (1) Lee,Gil Ho (1) Matsui, Takane (1) Morozumi, Tetsuo (1) Nakagawa, Michio (1) Yoe,Jeehyun (1) Yoe,Sung Moon (1) Yoshihara, Shinobu (1) アグリプリヨノ, デヴィ　ラテイ (1) 両角, 徹雄 (1) 中川, 迪夫 (1) 古岡, 秀文 (1) 吉原, 忍 (1) 松井, 高峯 (1) Mais... Palavra-chave Inclusion body (2) Adenovirus (1) Antibacterial activity (1) Encephalitis (1) Lysozyme (1) Nephritis (1) On-column refolding (1) Recombinant expression (1) Spodoptera litura (1) Swine (1) Mais... Tipo do documento Journal article (1) Ano 2011 (1) 1997 (1) País Chile (1) Japan (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Pathology of naturally occurring porcine adenovirus type 4 infection in Japan OAK Agungpriyono, Dewi Ratih; Nakagawa, Michio; Morozumi, Tetsuo; Yoshihara, Shinobu; Furuoka, Hidefumi; Matsui, Takane; アグリプリヨノ, デヴィ　ラテイ; 中川, 迪夫; 両角, 徹雄; 吉原, 忍; 古岡, 秀文; 松井, 高峯. Palavras-chave: Adenovirus; Encephalitis; Inclusion body; Nephritis; Swine. Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1326 Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli Electron. J. Biotechnol. Kim,Jong-Wan; Yoe,Jeehyun; Lee,Gil Ho; Yoe,Sung Moon. The chicken-type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an anti-bacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C-terminal fusion protein with glutathione S-transferase (GST) in Rosetta(DE3) Singles. The protein was produced as an inclusion body which was solubilized in 8 M urea, renatured by on-column refolding, and purified by reversed-phase chromatography to 95% purity. The purified rSLLyz demonstrated antibacterial activity against B. megaterium confirmed by inhibition zone assay. The overexpression and refolding strategy described in this study will provide a reliable technique for... Tipo: Journal article Palavras-chave: Antibacterial activity; Inclusion body; Lysozyme; On-column refolding; Recombinant expression; Spodoptera litura. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300006 Registros recuperados: 2 Primeira ... 1 ... Última

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